HUMAN ANATOMY - 180 DAY EBOOK
HUMAN ANATOMY - 180 DAY EBOOK
5th Edition
ISBN: 9781260942767
Author: McKinley
Publisher: Macmillan Higher Education
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Chapter 15, Problem 3MC
Summary Introduction

Introduction:

The central nervous system of the body includes the brain and spinal cord. The brain is the center for regulation of all kind of information received by the body. Different kinds of information is passed from the spinal cord to the brain. After processing, the response is generated by the brain.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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