Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 15, Problem 20Q
When PCR is performed “by hand” (not with a prepackaged test kit), the lab environment has to be scrupulously clean. Explain why that might be.
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Question #3:
In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would
transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming
there was just one gene conferring gametophytic obligate apomixis, and that the two parents are
inbreds, what would be the consequences of such a cross if:
a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non-
apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic
and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female
first (left side) in the parental cross.
b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non-
apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic
and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female
first (left side) in the…
Question #5:
Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The
genes show independent assortment. Recessive alleles at both loci are required for the facultative
apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C.
At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype.
A facultative apomict male, capable of producing viable pollen, was crossed with a sexually
reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic
ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following
temperatures*:
a) 15°C?
b) 25°C?
*for full credit, show crosses and genotypes where appropriate. Remember to position the
female first (left side) in the cross.
Type your answer here:
a. What percentage of a drug is eliminated after 4 half-lives? Please round to the nearest percent.
b. What will happen to elimination of the drug in the previous question if the system is saturated? explain and show any math involved
Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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