Nutrient gelatin can be incubated at 35°C. What would you have to do to determine hydrolysis after incubation at 35°C?
To determine:
The way by which hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined.
Introduction:
Gelatin is colorless and flavorless ingredient in food that is formed from collagen. In dry state it is brittle, while in liquid state it is kind of gummy. It is commonly used in medicines, paper, cosmetics, vitamin capsules, and photographic films.
Explanation of Solution
When gelatin is incubated at 35 degree Celsius, then to determine the hydrolysis after 35 degrees Celsius, the test tubes containing gelatin must be kept in refrigerator or in ice tub. As it is done, the unhydrolyzed gelatin solidifies again and the hydrolyzed gelatin remains in liquid state.
The hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined by keeping the tubes at low temperatures.
Want to see more full solutions like this?
Chapter 15 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
- How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?arrow_forwardConsider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…arrow_forwardWhat types of macromolecules are usually separated on agarose electrophoresis gels?arrow_forward
- Why do we need to place the lipid samples under direct heat after adding ethanol in a solubility test?arrow_forwardIn SDS polyacrylamide gel electrophoresis, which of the following polypeptides move slowest?A) The ones with the lowest molecular weightB) The ones with the highest molecular weightC) The most negatively chargedD) The most positively chargedE) The ones with the lowest buoyant densityarrow_forwardCan Benedicts solution be used distinguish between glucose and maltose solutions?arrow_forward
- Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.arrow_forwardA solution containing egg albumin (pl=4.6), B-lactoglobulin (pl=5.2), and chymotrypsinogen (pl=9.5) was loaded onto a column of diethylaminoethyl cellulose (DEAE-cellulose) at pH 5.4.The column was then eluted with a pH 5.4 buffer, with an increasing salt concentration. Predict the elution pattern.arrow_forwardIn a gel filtration chromatography, what type of gel must be used when the protein size is 2500 Da? Explain.arrow_forward
- What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm with the presence of the Bradford reagent? (note: explain not less than 5 sentences).arrow_forwardwhy do proteins get denatured under low temperatures? explain thoroughlyarrow_forwardA mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education