Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 15, Problem 1Q

Nutrient gelatin can be incubated at 35°C. What would you have to do to determine hydrolysis after incubation at 35°C?

Expert Solution & Answer
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Summary Introduction

To determine:

The way by which hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined.

Introduction:

Gelatin is colorless and flavorless ingredient in food that is formed from collagen. In dry state it is brittle, while in liquid state it is kind of gummy. It is commonly used in medicines, paper, cosmetics, vitamin capsules, and photographic films.

Explanation of Solution

When gelatin is incubated at 35 degree Celsius, then to determine the hydrolysis after 35 degrees Celsius, the test tubes containing gelatin must be kept in refrigerator or in ice tub. As it is done, the unhydrolyzed gelatin solidifies again and the hydrolyzed gelatin remains in liquid state.

Conclusion

The hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined by keeping the tubes at low temperatures.

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