Nutrient gelatin can be incubated at 35°C. What would you have to do to determine hydrolysis after incubation at 35°C?
To determine:
The way by which hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined.
Introduction:
Gelatin is colorless and flavorless ingredient in food that is formed from collagen. In dry state it is brittle, while in liquid state it is kind of gummy. It is commonly used in medicines, paper, cosmetics, vitamin capsules, and photographic films.
Explanation of Solution
When gelatin is incubated at 35 degree Celsius, then to determine the hydrolysis after 35 degrees Celsius, the test tubes containing gelatin must be kept in refrigerator or in ice tub. As it is done, the unhydrolyzed gelatin solidifies again and the hydrolyzed gelatin remains in liquid state.
The hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined by keeping the tubes at low temperatures.
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Chapter 15 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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