Which type of nutrient medium would a double mutant with argG and argE grow on. Introduction: Beadle and Tatum selected Neurospora crassa , the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Answer 6

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Answer 7

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Answer 8

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Answer 9

Expert Solution

Answer 10

Expert Solution

Answer 11

Expert Solution

Answer 12

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Answer 13

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Answer 14

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.