GENETICS(LL)-W/CONNECT >CUSTOM<
6th Edition
ISBN: 9781260571561
Author: HARTWELL
Publisher: MCG CUSTOM
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Chapter 15, Problem 16P
Suppose you are examining a newly found plant species, and you want to determine whether the inheritance of mtDNA is maternal, paternal, or biparental. You find that in the population two variants of mtDNA exist that can be distinguished by size differences of PCR amplification products made with a particular pair of primers. You first perform PCR analysis on DNA isolated from a leaf on each of two individual plants. Then you cross eggs from plant 1 with pollen from plant 2, obtain four seedlings, and perform PCR analysis with DNA from each of the whole seedlings. The results are shown below.
| a. | Assuming plant 2 is homoplasmic, do these results exclude any of the three possible models for the inheritance of mtDNA in this species (maternal, paternal, or biparental)? |
| b. | What experiment could you perform to distinguish between the models that remain? |
| c. | What experiment(s) could you perform to check your assumption that plant 2 is in fact homoplasmic? Why is such an experiment necessary to make a conclusion about the mode of inheritance of mtDNA in this species? |
| d. | Explain the differing proportions of the two forms of mtDNA in the four seedlings. Be as specific as possible. |
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The table below shows the results of an investigation in which the effect of
temperature and light on the yield of tomatoes in two greenhouses on a farm
was investigated.
TEMPERATURE
(°C)
AVERAGE YIELD OF TOMATOES PER
3.1
PLANT
(kg)
LOW LIGHT LEVELS
HIGH LIGHT LEVELS
5
0,5
0,5
10
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(2)
3.2
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3.3
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3.4
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Explain why you chose this mutation. Begin by transcribing and translating BOTH the
normal and abnormal DNA sequences. The genetic code below is for your reference.
SECOND BASE OF CODON
כ
FIRST BASE OF CODON
O
THIRD BASE OF CODON
SCAGUCAGUGAGUCAG
UUU
UUC
UCU
UAU
UGU
Phenylalanine
(F)
Tyrosine (Y)
Cysteine (C)
UCC
UAC
UGC
Serine (S)
UUA
UUG
Leucine (L)
UCA
UCG_
UAA
UGA
Stop codon
-Stop codon
UAG
UGG -Tryptophan (W)
CUU
CUC
CCU
CAU
CGU
Histidine (H)
CCC
CAC
CGC
-Leucine (L)
Proline (P)
CUA
CCA
CAA
CUG
CCG
CAG-Glutamine (Q)
-Arginine (R)
CGA
CGG
AUU
ACU
AAU
AGU
AUC
Isoleucine (1)
Asparagine (N)
ACC
AAC
Threonine (T)
AUA
ACA
AAA
Methionine (M)
Lysine (K)
AUG
ACG
Start codon
AAG
AGC-Serine (S)
-Arginine (R)
AGA
AGG
GUU
GCU
GAU
GUC
GUA
GUG
GCC
Valine (V)
-Alanine (A)
GCA
GCG
GAC
GAA
GAG
Aspartic acid
(D)
GGU
Glutamic acid
(E)
GGC
GGA
GGG
Glycine (G)
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Chapter 15 Solutions
GENETICS(LL)-W/CONNECT >CUSTOM<
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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