Bundle: Biology: Concepts and Applications, Loose-leaf Version, 10th + LMS Integrated MindTap Biology, 1 term (6 months) Printed Access Card
10th Edition
ISBN: 9781337538305
Author: Cecie Starr, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 15, Problem 11SA
Put the following tasks in the order they would occur during a
| a. using
b. using a probe to identify a clone in the library c. sequencing the d. making a e. cutting genomic |
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Chapter 15 Solutions
Bundle: Biology: Concepts and Applications, Loose-leaf Version, 10th + LMS Integrated MindTap Biology, 1 term (6 months) Printed Access Card
Ch. 15 - ________ cuts DNA molecules at specific sites. a....Ch. 15 - A _______ is a molecule that can be used to carry...Ch. 15 - Reverse transcriptase assembles an ________ on an...Ch. 15 - For each species, all ______ in the complete set...Ch. 15 - A set of cells that host various DNA fragments...Ch. 15 - Prob. 6SACh. 15 - Electrophoresis separates fragments of DNA...Ch. 15 - PCR can be used ______. a. as a cloning vector b....Ch. 15 - An individuals set of unique _______ can be used...Ch. 15 - Taq polymerase is used for PCR because it _______...
Ch. 15 - Put the following tasks in the order they would...Ch. 15 - True or false? Some transgenic organisms can pass...Ch. 15 - _______ can correct a genetic defect in an...Ch. 15 - True or false? Some humans are genetically...Ch. 15 - Match each term with the most suitable...Ch. 15 - Restriction enzymes in bacterial cytoplasm cut...Ch. 15 - The results of a paternity test are shown in the...
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- What did the Cre-lox system used in the Kikuchi et al. 2010 heart regeneration experiment allow researchers to investigate? What was the purpose of the cmlc2 promoter? What is CreER and why was it used in this experiment? If constitutively active Cre was driven by the cmlc2 promoter, rather than an inducible CreER system, what color would you expect new cardiomyocytes in the regenerated area to be no matter what? Why?arrow_forwardWhat kind of organ size regulation is occurring when you graft multiple organs into a mouse and the graft weight stays the same?arrow_forwardWhat is the concept "calories consumed must equal calories burned" in regrads to nutrition?arrow_forward
- You intend to insert patched dominant negative DNA into the left half of the neural tube of a chick. 1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side? 2) What would be the internal (within the embryo) control for this experiment? 3) How can you be sure that the electroporation method itself is not impacting the embryo? 4) What would you do to ensure that the electroporation is working? How can you tell?arrow_forwardDescribe a method to document the diffusion path and gradient of Sonic Hedgehog through the chicken embryo. If modifying the protein, what is one thing you have to consider in regards to maintaining the protein’s function?arrow_forwardThe following table is from Kumar et. al. Highly Selective Dopamine D3 Receptor (DR) Antagonists and Partial Agonists Based on Eticlopride and the D3R Crystal Structure: New Leads for Opioid Dependence Treatment. J. Med Chem 2016.arrow_forward
- The following figure is from Caterina et al. The capsaicin receptor: a heat activated ion channel in the pain pathway. Nature, 1997. Black boxes indicate capsaicin, white circles indicate resinferatoxin. You are a chef in a fancy new science-themed restaurant. You have a recipe that calls for 1 teaspoon of resinferatoxin, but you feel uncomfortable serving foods with "toxins" in them. How much capsaicin could you substitute instead?arrow_forwardWhat protein is necessary for packaging acetylcholine into synaptic vesicles?arrow_forward1. Match each vocabulary term to its best descriptor A. affinity B. efficacy C. inert D. mimic E. how drugs move through body F. how drugs bind Kd Bmax Agonist Antagonist Pharmacokinetics Pharmacodynamicsarrow_forward
- 50 mg dose of a drug is given orally to a patient. The bioavailability of the drug is 0.2. What is the volume of distribution of the drug if the plasma concentration is 1 mg/L? Be sure to provide units.arrow_forwardDetermine Kd and Bmax from the following Scatchard plot. Make sure to include units.arrow_forwardChoose a catecholamine neurotransmitter and describe/draw the components of the synapse important for its signaling including synthesis, packaging into vesicles, receptors, transporters/degradative enzymes. Describe 2 drugs that can act on this system.arrow_forward
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