Biology
Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 14.4, Problem 1TC

For any single person, a given STR always has either one or two bands. Why? Further, single bands are always about twice as bright as each band of a pair. For example, in the D16 STR on the right, the single bands of the first and third DNA samples are twice as bright as the pairs of bands of the second, fourth, and fifth samples. Why?

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Based from Fig. 1B, which of the following lanes contain a good quality genomic DNA? Bad quality genomic DNA? Why or Why not? Answer in maximum of 5 sentences only. You can prove your answer by describing the banding pattern of each lanes and how to troubleshoot the DNA extraction procedure if possible.
Listed below are 4 of the 13 genome sites used to create a standard DNA profile. Each site consists of a number of short tandem repeats: sets of 4 nucleotides repeated in a row within the genome. For each site, the number of repeats found at that site for this individual are listed: Imagine you perform a PCR procedure to create a DNA profile for this individual. Which of the following four gels correctly represents the DNA profile of this person?
How would you estimate the size of the unknown DNA fragement just by looking at the gel?

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