The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Answer 3

Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Answer 4

Question

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

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Answer 5

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 6

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 7

Chapter 14, Problem 4IQ

Summary Introduction

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Answer 8

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Answer 11

Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LO Ch. 14.3 - Prob. 1LO Ch. 14.3 - Prob. 2LO Ch. 14.4 - Prob. 1LO Ch. 14.4 - Prob. 2LO Ch. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.

The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Solution Summary: The author explains the phenotype of Dam mutants. The enzyme adenine methylase works in the 5’-GATC-3’ direction.

Concept explainers

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To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Want to see the full answer?

Chapter 14 Solutions

The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Solution Summary: The author explains the phenotype of Dam mutants. The enzyme adenine methylase works in the 5’-GATC-3’ direction.

Concept explainers

Videos

To determine: The phenotype of Dam mutants. Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.

Want to see the full answer?

Chapter 14 Solutions

To determine: The phenotype of Dam mutants.

Introduction: The enzyme adenine methylase is responsible for adding methyl groups to the DNA strand. The enzyme works in the 5’-GATC-3’ to the newly synthesized DNA strands.