Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 14, Problem 4AYKB
Many chemical warfare agents, such as the poisonous gas sarin, block the enzyme acetylcholinesterase in the synaptic cleft. What effects will this poison have on muscle contraction? (Connects toChapter 10) What effects will it have on the autonomic nervous system? What symptoms would you expect to see from this poison?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 14 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 14.1 - What are the basic functions of the ANS?Ch. 14.1 - 2. What are autonomic ganglia?
Ch. 14.1 - How do somatic motor neurons and autonomic neurons...Ch. 14.1 - 4. What are preganglionic and postganglionic...Ch. 14.1 - What are the two divisions of the ANS?Ch. 14.1 - How are the two divisions of the ANS alike, and...Ch. 14.2 - 1. Where are the cell bodies of sympathetic...Ch. 14.2 - Where are the cell bodies of sympathetic...Ch. 14.2 - Which neurotransmitter(s) is (are) released by...Ch. 14.2 - Prob. 4QC
Ch. 14.2 - Describe the effects of the sympathetic nervous...Ch. 14.2 - Prob. 6QCCh. 14.3 - What is the basic function of the parasympathetic...Ch. 14.3 - Where are the preganglionic parasympathetic cell...Ch. 14.3 - How does the arrangement of neurons in the...Ch. 14.3 - 4. Which neurotransmitter is released by all...Ch. 14.3 - Prob. 5QCCh. 14.3 - Prob. 6QCCh. 14.3 - Summarize the effects of the parasympathetic...Ch. 14.4 - Prob. 1QCCh. 14.4 - Prob. 2QCCh. 14.4 - 3. What is autonomic tone?
Ch. 14.4 - How does the CNS mediate control over the ANS?Ch. 14 - Which of the following best describes the basic...Ch. 14 - 2. Fill in the blanks: The sympathetic nervous...Ch. 14 - 3. Sympathetic preganglionic neurons synapse:
a....Ch. 14 - Differentiate between adrenergic and cholinergic...Ch. 14 - Prob. 5CYRCh. 14 - Explain what happens when sympathetic neurons...Ch. 14 - Prob. 7CYRCh. 14 - Mark each of the following as an effect of the...Ch. 14 - Mark the following statements as true or false. If...Ch. 14 - 10. Fill in the blanks: _______receptors are...Ch. 14 - Parasympathetic ganglia are typically: a. located...Ch. 14 - Central nervous system control over the ANS is...Ch. 14 - 1. Using 20 or fewer words, define each of the...Ch. 14 - You are running a race to the top of a mountain....Ch. 14 - 3. Describe all the changes initiated by the...Ch. 14 - 1. Which cranial nerves would be affected by a...Ch. 14 - 2. A patient, Dr. Young, has both asthma and high...Ch. 14 - Mr. Chevalier has been diagnosed with Horner...Ch. 14 - Many chemical warfare agents, such as the...
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