
(a)
To determine: The reason that why it is simpler strategy than the reverse engineering E. coli to be more ethanol tolerant.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This condition occurs in the absence of oxygen. In humans, the process of fermentation relies on the first stage that is glycolysis of the
(b)
To determine: The chemical transformation catalyzed by each of three ara enzymes.
Introduction:
The ara genes produce enzymes that convert L-arabinose into L-xylulose-5-phosphate. This is a multistep reaction which includes three different enzymes encoded by different ara genes.
(c)
To determine: The name of the sugar which is converted from arabinose by three ara enzymes.
Introduction:
The ara genes produce three different enzymes encoded by different ara genes that is used to convert L-arabinose .
(d)
To describe: The overall pathway for the fermentation of six molecules of arabinose to ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a
(e1)
To determine: The stoichiometry of the fermentation of six molecules of arabinose to ethanol and CO2.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(e2)
To determine: The number of ATP molecules that would be generated in this reaction.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(f)
To explain: The reason that production of 1 ATP per molecule of arabinose is less beneficial for bacterium but better for production of ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(g)
To determine: The additional enzymes that would allow Z. mobilis to use xylose as well as arabinose to produce ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.

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Chapter 14 Solutions
Lehninger Principles of Biochemistry
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- Four distinct classes of interactions (inter and intramolecular forces) contribute to a protein's tertiary and quaternary structures. Name the interaction then describe the amino acids that can form this type of interaction. Draw and annotate a diagram of the interaction between two amino acids.arrow_forwardExamine the metabolic pathway. The enzymes that catalyze each step are identified as "e" with a numeric subscript. e₁ e3 e4 A B с 1° B' 02 e5 e6 e7 E F Which enzymes catalyze irreversible reactions? ப e ez ☐ ez e4 ☐ ப es 26 5 e7 Which of the enzymes is likely to be the allosteric enzyme that controls the synthesis of G? €2 ез e4 es 26 5 e7arrow_forwardAn allosteric enzyme that follows the concerted model has an allosteric coefficient (T/R) of 300 in the absence of substrate. Suppose that a mutation reversed the ratio. Select the effects this mutation will have on the relationship between the rate of the reaction (V) and substrate concentration, [S]. ㅁㅁㅁ The enzyme would likely follow Michaelis-Menten kinetics. The plot of V versus [S] would be sigmoidal. The enzyme would mostly be in the T form. The plot of V versus [S] would be hyperbolic. The enzyme would be more active.arrow_forward
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