(a)
To determine: The reason that why it is simpler strategy than the reverse engineering E. coli to be more ethanol tolerant.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This condition occurs in the absence of oxygen. In humans, the process of fermentation relies on the first stage that is glycolysis of the
(b)
To determine: The chemical transformation catalyzed by each of three ara enzymes.
Introduction:
The ara genes produce enzymes that convert L-arabinose into L-xylulose-5-phosphate. This is a multistep reaction which includes three different enzymes encoded by different ara genes.
(c)
To determine: The name of the sugar which is converted from arabinose by three ara enzymes.
Introduction:
The ara genes produce three different enzymes encoded by different ara genes that is used to convert L-arabinose .
(d)
To describe: The overall pathway for the fermentation of six molecules of arabinose to ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a
(e1)
To determine: The stoichiometry of the fermentation of six molecules of arabinose to ethanol and CO2.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(e2)
To determine: The number of ATP molecules that would be generated in this reaction.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(f)
To explain: The reason that production of 1 ATP per molecule of arabinose is less beneficial for bacterium but better for production of ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.
(g)
To determine: The additional enzymes that would allow Z. mobilis to use xylose as well as arabinose to produce ethanol.
Introduction:
“Anaerobic harvest” of food energy is known as fermentation. This is a metabolic process carried out mainly by yeast and some bacteria. They convert sugar into carbon dioxide and ethanol.

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Chapter 14 Solutions
Lehninger Principles of Biochemistry (Instructor's)
- A culture of kidneys cells contains all intermediates of the citric acid cycle. It is treated with an irreversible inhibitor of malate dehydrogenase, and then infused withglucose. Fill in the following list to account for the number of energy molecules that are formed from that one molecule of glucose in this situation. (NTP = nucleotidetriphosphate, e.g., ATP or GTP)Net number of NTP:Net number of NADH:Net number of FADH2:arrow_forward16. Which one of the compounds below is the final product of the reaction sequence shown here? OH A B NaOH Zn/Hg aldol condensation heat aq. HCI acetone C 0 D Earrow_forward2. Which one of the following alkenes undergoes the least exothermic hydrogenation upon treatment with H₂/Pd? A B C D Earrow_forward
- 6. What is the IUPAC name of the following compound? A) (Z)-3,5,6-trimethyl-3,5-heptadiene B) (E)-2,3,5-trimethyl-1,4-heptadiene C) (E)-5-ethyl-2,3-dimethyl-1,5-hexadiene D) (Z)-5-ethyl-2,3-dimethyl-1,5-hexadiene E) (Z)-2,3,5-trimethyl-1,4-heptadienearrow_forwardConsider the reaction shown. CH2OH Ex. CH2 -OH CH2- Dihydroxyacetone phosphate glyceraldehyde 3-phosphate The standard free-energy change (AG) for this reaction is 7.53 kJ mol-¹. Calculate the free-energy change (AG) for this reaction at 298 K when [dihydroxyacetone phosphate] = 0.100 M and [glyceraldehyde 3-phosphate] = 0.00300 M. AG= kJ mol-1arrow_forwardIf the pH of gastric juice is 1.6, what is the amount of energy (AG) required for the transport of hydrogen ions from a cell (internal pH of 7.4) into the stomach lumen? Assume that the membrane potential across this membrane is -70.0 mV and the temperature is 37 °C. AG= kJ mol-1arrow_forward
- Consider the fatty acid structure shown. Which of the designations are accurate for this fatty acid? 17:2 (48.11) 18:2(A9.12) cis, cis-A8, A¹¹-octadecadienoate w-6 fatty acid 18:2(A6,9)arrow_forwardClassify the monosaccharides. H-C-OH H. H-C-OH H-C-OH CH₂OH H-C-OH H-C-OH H-C-OH CH₂OH CH₂OH CH₂OH CH₂OH D-erythrose D-ribose D-glyceraldehyde Dihydroxyacetone CH₂OH CH₂OH C=O Answer Bank CH₂OH C=0 HO C-H C=O H-C-OH H-C-OH pentose hexose tetrose H-C-OH H-C-OH H-C-OH aldose triose ketose CH₂OH CH₂OH CH₂OH D-erythrulose D-ribulose D-fructosearrow_forwardFatty acids are carboxylic acids with long hydrophobic tails. Draw the line-bond structure of cis-A9-hexadecenoate. Clearly show the cis-trans stereochemistry.arrow_forward
- The formation of acetyl-CoA from acetate is an ATP-driven reaction: Acetate + ATP + COA Acetyl CoA+AMP+ PP Calculate AG for this reaction given that the AG for the hydrolysis of acetyl CoA to acetate and CoA is -31.4 kJ mol-1 (-7.5 kcal mol-¹) and that the AG for hydrolysis of ATP to AMP and PP; is -45.6 kJ mol-1 (-10.9 kcal mol-¹). AG reaction kJ mol-1 The PP, formed in the preceding reaction is rapidly hydrolyzed in vivo because of the ubiquity of inorganic pyrophosphatase. The AG for the hydrolysis of pyrophosphate (PP.) is -19.2 KJ mol-¹ (-4.665 kcal mol-¹). Calculate the AG° for the overall reaction, including pyrophosphate hydrolysis. AGO reaction with PP, hydrolysis = What effect does the presence of pyrophosphatase have on the formation of acetyl CoA? It does not affect the overall reaction. It makes the overall reaction even more endergonic. It brings the overall reaction closer to equilibrium. It makes the overall reaction even more exergonic. kJ mol-1arrow_forwardConsider the Haworth projections of ẞ-L-galactose and ẞ-L-glucose shown here. OH CH₂OH OH CH₂OH OH OH OH ОН OH он B-L-galactose B-L-glucose Which terms describe the relationship between these two sugars? epimers enantiomers anomers diastereomersarrow_forwardClassify each characteristic as describing anabolism or catabolism. Anabolism Answer Bank Catabolism transforms fuels into cellular energy, such as ATP or ion gradients uses NADPH as the electron carrier synthesizes macromolecules requires energy inputs, such as ATP uses NAD+ as the electron carrier breaks down macromoleculesarrow_forward
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