Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 2CT
How could you determine which of the following carbohydrates a bacterium fermented: mannitol, sorbitol, adonitol, or arabinose?
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You are given a mixed culture of 3 bacteria with following characteristics-
1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4
sugars but forms endospores and is sensitive to penicillin)
2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce
carbon dioxide gas on fermenting these
3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose
and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.)
Also they all give similar looking white-colored colonies on an agar plate.
Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools
that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as
confirm them. Any other technique that you use should be one you learned in lab. No need to give
detailed info about how to perform the technique but it…
Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most microorganisms. Why does this property make agar an excellent solidifying agent for microbiological media?
A bacterial organism is used to inoculate mannitol broth medium. Following incubation the broth is yellow. This indicates that the organism:
A) can use lactose and glucose
B) cannot use lactose
C) can use mannitol
D) cannot use mannitol
Chapter 14 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 14 - Why are fermentation tubes evaluated at 24 and 48...Ch. 14 - If an organism metabolizes glucose aerobically,...Ch. 14 - Which of these media is selective?...Ch. 14 - Could an organism be a fermenter and also be both...Ch. 14 - How could you determine which of the following...Ch. 14 - If a bacterium cannot ferment glucose, why not...Ch. 14 - If a bacterium cannot use glucose, what result...Ch. 14 - If a bacterium cannot use citrate, what result...Ch. 14 - You put an opened bottle of orange drink in the...
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