BIOLOGY 12E CONNECT ACCESS CARD
12th Edition
ISBN: 9781264938513
Author: Raven
Publisher: MCG
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Textbook Question
Chapter 14, Problem 2A
Hershey and Chase used radioactive phosphorus and sulfur to
a. label DNA and protein uniformly.
b. differentially label DNA and protein.
c. identify the transforming principle.
d. Both b and c are correct.
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Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.
B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube.
D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?
a.
Chain termination occurs during synthesis of a new DNA strand.
b.
Many steps can be automated.
c.
No DNA is synthesized in the procedure.
d.
Fluorescent molecules can be used to detect the DNA.
Which of the following is true?
a.
In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore.
b.
Next generation sequencing is slow but accurate.
c.
Both of the above are true.
Chapter 14 Solutions
BIOLOGY 12E CONNECT ACCESS CARD
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardWhich of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardAvery, MacLeod, and McCarty demonstrated that the transforming principle can be destroyed by DNase. Thus, DNA is the genetic material A. Both statements are correct B. Both statements are incorrect C. First statement is Correct, Second is Incorrect D. First statement is Incorrect, Second is Correctarrow_forward
- The in situ hybridization method is based on Select one: a. labeling specific neuronal components with antibodies b. injecting a fluorescent dye into a neuron c. nucleic acid chains complementary to mRNAs that encode specific genes d. using nucleic acid probes to detect specific proteins e. formation of an insoluble colored product within cell bodiescarrow_forwardThe ab initio approach finds genes by looking for a. common sequences found in most genes. b. similarity in sequence with known genes. c. mRNA with the use of in situ hybridization. d. mutant phenotypesarrow_forwardThe Hershey-Chase experiment, in which bacteria were infected with radioactively labeled bacterial virus, provided evidence that DNA carries genetic information by showing that A. the bacterial virus injected its 35S-labeled proteins into the cell. B. blender treatment after infection with virus prevented the production of progeny virus particles. C. the production of progeny virus required the injection of viral DNA into the cell. D. 32P-labeled bacterial virus could not produce progeny virus after blender treatment.arrow_forward
- With the action of detergents learned from this experiment, describe the potential harm to aquatic organisms of the practice of disposing large amounts of detergent into bodies of water.arrow_forwardWhich of the following is NOT used in an in situ hybridization experiment for visualizing DNA? (CSLO 1, CSLO 3, CSLO 4, CSLO 7) O A. chromosome spread O B. probe O C. denaturation solution D. antibody E. microscopearrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
- Which of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forwardGel electrophoresis can be used to separate DNA on the basis of: A. the probe used. B. size. C. G and C nucleotide content. D. both size and loading buffer content. E. loading buffer content.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
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