BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
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Chapter 14, Problem 22P
Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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