Chapter 14, Problem 14.38P

Interpretation Introduction

Interpretation:

The protein (P) gives a three type of cluster and what mass-charge values do these clusters appear in the mass spectrum, the correct reason has to be explained with the given molecular mass values.

Concept Introduction:

Electrospray ionization (ESI):

• • This technique used in mass spectrometry to produced different ions.
• • It is especially useful in producing ions from macromolecules because it overcomes the propensity of these molecules to fragment when ionized.
• • The primary ion source used in liquid chromatography-mass spectrometry, because it is liquid-gas interface that is capable of coupling liquid chromatography with mass spectrometry. 

Mass spectrum: It generates multiple ions from the sample under investigation, it than separates them according to their specific mass to charge ration $\left(m/z\right)$ and records the relative abundance (RA) of each ion types.

      $\begin{array}{l}\text{M}\stackrel{\begin{array}{l}\text{electron}\\ \text{beam}\end{array}}{\to }{\text{M}}^{\begin{array}{l}+\\ .\end{array}}+{\text{e}}^{\text{-}}\\ \text{[Molecule]}\text{Molecular ion}\text{electron}\\ [\text{Radical}\text{cation}]\end{array}$