Chapter 13, Problem 5EQ

Summary Introduction

To review:

The estimated results of the Western blot technique for the given samples.

Lane 1: Proteins that are extracted from muscle cells.

Lane 2: Proteins that have been derived from nerve cells.

Lane 3: Proteins that are isolated from the muscle cells of a person who is homozygous for the mutation, which causes the introduction of a stop codon at codon 177 in the gene that encodes the protein troponin.

Introduction:

Western blotting is a technique which is used for finding the protein which is translated from a specific messenger ribonucleic acid (mRNA). It is a technique which involves the detection of polypeptide using an antibody which particularly recognizes and binds to the amino acid sequence of that protein.