Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 13, Problem 5AYKB
Another feature of CIPA is anhidrosis, or the inability to sweat. Explain why Maria’s inability to produce sweat could potentially be life-threatening. (Connects toChapter 5)
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 13 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 13.1 - What two subclasses make up the sensory division...Ch. 13.1 - 2. What is a lower motor neuron? How are upper...Ch. 13.1 - In what ways do the somatic and visceral motor...Ch. 13.1 - Prob. 4QCCh. 13.1 - Prob. 5QCCh. 13.1 - What structures are found in a peripheral nerve?Ch. 13.1 - How are sensations detected in the PNS and...Ch. 13.1 - 8. How are motor impulses transmitted from the...Ch. 13.2 - Prob. 1QCCh. 13.2 - 2. What are the Roman numerals and main...
Ch. 13.2 - 3. What are the Roman numerals and main...Ch. 13.2 - List the 12 pairs of cranial nerves in ascending...Ch. 13.2 - Prob. 5QCCh. 13.3 - Prob. 1QCCh. 13.3 - What are the anterior and posterior rami, and what...Ch. 13.3 - 3. What are the key structures supplied by each...Ch. 13.3 - 4. Differentiate between the trunks and cords of...Ch. 13.3 - Prob. 5QCCh. 13.3 - Prob. 6QCCh. 13.3 - Prob. 7QCCh. 13.4 - 1. What is sensory transduction?
Ch. 13.4 - Prob. 2QCCh. 13.4 - 3. What are the three components of a typical...Ch. 13.4 - What is a first-order sensory neurons receptive...Ch. 13.4 - What is the two-point discrimination threshold,...Ch. 13.4 - What is a dermatome?Ch. 13.4 - 7. Why is visceral pain often perceived as...Ch. 13.5 - 1. What are the main differences between an upper...Ch. 13.5 - 2. What is a motor neuron pool?
Ch. 13.5 - What is the general sequence of events for...Ch. 13.6 - Prob. 1QCCh. 13.6 - 2. How do intrafusal and extrafusal muscle fibers...Ch. 13.6 - What are the functions of primary and secondary...Ch. 13.6 - 4. How do Golgi tendon organs and muscle spindles...Ch. 13.6 - How do polysynaptic and monosynaptic reflex arcs...Ch. 13.6 - Prob. 6QCCh. 13.6 - How are the flexion and crossed-extension reflexes...Ch. 13.6 - What are some potential effects of sensory...Ch. 13.6 - How do upper and lower motor neuron disorders...Ch. 13 - Mark the following statements as true or false. If...Ch. 13 - Prob. 2CYRCh. 13 - 3. Define each of the following terms in your own...Ch. 13 - First, write the Roman numeral that corresponds to...Ch. 13 - Prob. 5CYRCh. 13 - Match the following nerves with the structures...Ch. 13 - First-order somatic sensory neurons are...Ch. 13 - Prob. 8CYRCh. 13 - Prob. 9CYRCh. 13 - 10. Merkel cell fibers, tactile corpuscles,...Ch. 13 - 11. Place the following sequence of events for the...Ch. 13 - How do upper and lower motor neurons differ?Ch. 13 - 13. List and describe the basic steps involved in...Ch. 13 - 14. The lower motor neurons that innervate...Ch. 13 - Fill in the blanks:______ detect the degree to...Ch. 13 - Which of the following is the correct order of...Ch. 13 - 17. Mark the following statements as true or...Ch. 13 - Prob. 18CYRCh. 13 - Prob. 1CYUCh. 13 - Prob. 2CYUCh. 13 - Prob. 3CYUCh. 13 - Prob. 1AYKACh. 13 - Jason presents for evaluation after a severe...Ch. 13 - 3. When Mr. Williams goes to the emergency...Ch. 13 - 4. Maria is a 3-year-old who has been diagnosed...Ch. 13 - Another feature of CIPA is anhidrosis, or the...
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