Chapter 13, Problem 57E.3RQ

a.

Summary Introduction

To explain: The rationale for using EDTA in plasmid isolation.

Introduction: The plasmids are most commonly found in bacteria. The isolation of plasmid from the bacterial cells is a multistep process. The steps involved in the plasmid isolation are rupturing a bacterium that bears plasmid, removal of cellular components using various reagents, and finally, the plasmid DNA is suspended in an aqueous solution.

b.

Summary Introduction

To explain: The rationale for using sodium dodecyl sulfate (SDS) in plasmid isolation.

c.

Summary Introduction

To explain: The rationale for using potassium acetate in plasmid isolation.