Biology: Concepts and Investigations
5th Edition
ISBN: 9781260259049
Author: Hoefnagels
Publisher: MCG
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Chapter 1.3, Problem 2MC
Summary Introduction
To explain:
The definition of a statistically significant result.
Concept Introduction:
The experiment includes the prediction of hypothesis and verification of the results. The probability or the chance of the event is analyzed and calculated. The samples are collected from the surroundings and the experiments are conducted to check the dependency of the results on the random and dependent variables.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 1 Solutions
Biology: Concepts and Investigations
Ch. 1.1 - Does any nonliving object possess all of the...Ch. 1.1 - Prob. 2MCCh. 1.1 - The bacteria in figure 1.8 reproduce asexually,...Ch. 1.2 - What are the goals of taxonomy?Ch. 1.2 - How are domains related to kingdoms?Ch. 1.2 - Prob. 3MCCh. 1.3 - Identify the elements of the experiment summarized...Ch. 1.3 - Prob. 2MCCh. 1.3 - What is the difference between a hypothesis and a...Ch. 1.3 - Prob. 4MC
Ch. 1.3 - Compare and contrast science and technology.Ch. 1.4 - Prob. 1MCCh. 1.4 - How does this story illustrate discovery science?Ch. 1 - Which of the following is smaller than an...Ch. 1 - All of the following are characteristics of life...Ch. 1 - The concentration of salts in blood remains...Ch. 1 - Because plants extract nutrients from soil and use...Ch. 1 - Evolution through natural selection will occur...Ch. 1 - Prob. 6MCQCh. 1 - In an experiment to test the effect of temperature...Ch. 1 - A scientist has just observed a new phenomenon and...Ch. 1 - Prob. 9MCQCh. 1 - Describe each of the five characteristics of life,...Ch. 1 - Imagine two related species of single-celled...Ch. 1 - Prob. 3WIOCh. 1 - Think of an analogy that will help you remember...Ch. 1 - Other than the examples given in the text name an...Ch. 1 - Draw and explain the relationship between...Ch. 1 - Prob. 7WIOCh. 1 - Prob. 8WIOCh. 1 - How are the members of the three domains similar?...Ch. 1 - List each step of the scientific method and...Ch. 1 - Give two examples of questions that you cannot...Ch. 1 - Prob. 1PITCh. 1 - Prob. 2PITCh. 1 - Prob. 3PIT
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