BIOCHEMISTRY 2 TERM ACCESS
BIOCHEMISTRY 2 TERM ACCESS
9th Edition
ISBN: 9781319402877
Author: BERG
Publisher: MAC HIGHER
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Chapter 13, Problem 26P
Interpretation Introduction

Interpretation:

The reason for the efficiency of the enzyme to be physiologically crucial needs to be explained.

Concept introduction:

Acetylcholine act as a neurotransmitter which means it sends messages or chemicals from one cell to another cell of the body. Acetylcholinesterase helps in stopping the excitation of a nerve cell after the transmission of impulse or in other words, this enzyme causes rapid hydrolysis of acetylcholine.

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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).  Lane 1:  buffer Lane 2 : Ladder Lane 3: Lysate Lane 4: Flowthrough Lane 5: Wash Lanes 6-8: E1, E2, E3 Lane 9: Dialyzed VRK1  Lane 10: LDH
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You have isolated a protein and determined that the native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. Describe the architecture of the polypeptide component of this enzyme.
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