What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and then grown in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium. Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

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Amino Acid Coclow TABle 3' Gly Phe Leu (G) (F) (L) 3- Val (V) Arg (R) Ser (S) Ala (A) Lys (K) CAG G Glu Asp (E) (D) Ser (S) CCCAGUCAGUCAGUCAG 0204 C U A G C Asn (N) G 4 A AGU C GU (5) AC C UGA A G5 C CUGACUGACUGACUGAC Thr (T) Met (M) lle £€ (1) U 4 G Tyr Σε (Y) U Cys (C) C A G Trp (W) 3' U C A Leu בוט His Pro (P) ££ (H) Gin (Q) Arg 흐름 (R) (L) Start Stop 8. Transcription and Translation Practice: (Video 10-1 and 10-2) A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense DNA strand and label the 5' and 3' ends. B. Use the antisense strand that you create in part A as a template to create the mRNA transcript of the gene and label the 5' and 3' ends. C. Translate the mRNA you produced in part B into the polypeptide sequence making sure to follow all the rules of translation. 5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand) 4

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Chapter 13, Problem 17TYU

Summary Introduction

To explain: What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and then grown in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium.

Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

Summary Introduction

To explain: What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and if the fungus does not grow in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium.

Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

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