EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 12.5, Problem 1CR
Describe two widely used reporter genes.
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Students have asked these similar questions
9. Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two
fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure
below. Use the information below to determine how much ATP will be produced from the glycerol part of
the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally
put the NADH and ATP yields together from the glycerol and fatty acids (part A and B) to determine your
total number of ATP produced per lipid. Assume no other carbon source is available.
18 carbons
fatty acids
12 carbons
9
glycerol
A. Glycerol is broken down to glyceraldehyde 3-phosphate, a glycolysis intermediate via the following
pathway shown in the figure below. Notice this process costs one ATP but generates one FADH2. Continue
generating ATP with glyceraldehyde-3-phosphate using the standard pathway and aerobic respiration.
glycerol
glycerol-3-
phosphate…
Normal dive (for diving humans)
normal
breathing
dive
normal
breathing
Oz level
CO2 level
urgent need
to breathe
Oz blackout zone
high CO2 triggers breathing
6. This diagram shows rates of oxygen depletion and carbon dioxide accumulation in the blood in
relation to the levels needed to maintain consciousness and trigger the urgent need to breathe in
diving humans.
How might the location and slope of the O₂ line differ for diving marine mammals such as
whales and dolphins?
•
How might the location and slope of the CO₂ line differ for diving marine mammals such as
whales and dolphins?
•
•
Draw in predicted lines for O2 and CO2, based on your reasoning above.
How might the location of the Urgent Need to Breathe line and the O2 Blackout Zone line
differ for diving marine mammals?
What physiological mechanisms account for each of these differences, resulting in the ability
of marine mammals to stay submerged for long periods of time?
foraging/diet type
teeth
tongue
stomach
intestines
cecum
Insectivory
numerous, spiky, incisors procumbentExample: moleExample: shrew
--
simple
short
mostly lacking
Myrmecophagy
absent or reduced in numbers, peg-likeExample: tamandua anteater
extremely long
simple, often roughened
short
small or lacking
Terrestrial carnivory
sharp incisors; long, conical canines; often carnassial cheek teeth; may have crushing molarsExample: dog
--
simple
short
small
Aquatic carnivory
homodont, spiky, numerousExample: common dolphin
--
simple or multichambered (cetaceans only)
variable
small or absent
Sanguinivory
very sharp upper incisors; reduced cheek teethExample: vampire bat
grooved
tubular, highly extensible
long
small or lacking
Herbivory (except nectivores)
incisors robust or absent; canines reduced or absent; diastema; cheek teeth enlarged with complex occlusal surfacesExample: beaver
--
simple (hindgut fermenters) or multichambered (ruminants)
long
large
Filter feeding
none…
Chapter 12 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- 3. Shown below is the dental formula and digestive tract anatomy of three mammalian species (A, B, and C). What kind of diet would you expect each species to have? Support your answers with what you can infer from the dental formula and what you can see in the diagram. Broadly speaking, what accounts for the differences? Species A 3/3, 1/1, 4/4, 3/3 པར『ན་ cm 30 Species B 4/3, 1/1, 2/2, 4/4 cm 10 Species C 0/4, 0/0,3/3, 3/3 020arrow_forward3. Shown below is the dental formula and digestive tract anatomy of three mammalian species (A, B, and C). What kind of diet would you expect each species to have? Support your answers with what you can infer from the dental formula and what you can see in the diagram. Broadly speaking, what accounts for the differences? Species A 3/3, 1/1, 4/4, 3/3 cm 30 Species B 0/4, 0/0, 3/3, 3/3 cm 10 Species C 4/3, 1/1, 2/2, 4/4 E 0 cm 20 AILarrow_forwardNormal dive (for diving humans) normal breathing dive normal breathing Oz level CO₂ level urgent need to breathe Oz blackout zone high CO₂ triggers breathing 6. This diagram shows rates of oxygen depletion and carbon dioxide accumulation in the blood in relation to the levels needed to maintain consciousness and trigger the urgent need to breathe in diving humans. • How might the location and slope of the O2 line differ for diving marine mammals such as whales and dolphins? • How might the location and slope of the CO2 line differ for diving marine mammals such as whales and dolphins? • • Draw in predicted lines for O2 and CO2, based on your reasoning above. How might the location of the Urgent Need to Breathe line and the O2 Blackout Zone line differ for diving marine mammals? What physiological mechanisms account for each of these differences, resulting in the ability of marine mammals to stay submerged for long periods of time?arrow_forward
- How much ATP will be produced during the following metabolic scenario: Aerobic respiration of a 5mM lipid solution that is made up of one glycerol and an 8-carbon fatty acid and 12-carbon fatty acid. Recall that when glycerol breaks down to Glyceraldehyde-3-phosphate it costs one ATP but your get an extra FADH2. Every two carbons of a fatty acid break down to one acetyl-CoA. Units cannot be entered in this style of question but the units of your answer should be in mM of ATP.arrow_forwardIf a bacterium using aerobic respiration was to degrade one small protein molecule into 8 molecules of pyruvic acid, how many ATP would that cell make? Assume there is no other carbon source. Units cannot be entered in this style of question but the units of your answer should be in molecules of ATP.arrow_forwardIf a bacterium using aerobic respiration was to degrade a 30 mM solution of citric acid, how many ATP would that cell make? Assume no other carbon source is available. Units cannot be entered in this style of question but the units of your answer should be in mM of ATP.arrow_forward
- How much ATP will be produced during the following metabolic scenario: Aerobic respiration of a 5mM lipid solution that is made up of one glycerol and an 8-carbon fatty acid and 12-carbon fatty acid. Recall that when glycerol breaks down to Glyceraldehyde-3-phosphate it costs one ATP but your get an extra FADH2. Every two carbons of a fatty acid break down to one acetyl-CoA. (pathways will be provided on the exam) Units cannot be entered in this style of question but the units of your answer should be in mM of ATP.arrow_forwardWhen beta-lactamase was isolated from Staphylcoccus aureus and treated with a phosphorylating agent, only the active site, serine was phosphorylated. Additionally, the serine was found to constitute 0.35% (by weight) of this beta-lactamase enzyme. Using this, calculate the molecular weight of this enzyme and estimate the number of amino acids present in the polypeptide.arrow_forwardBased on your results from the Mannitol Salt Agar (MSA) media, which of your bacteria were mannitol fermenters and which were not mannitol fermenters?arrow_forward
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