EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 12.4, Problem 1MQ
- How can site-directed mutagenesis be useful to enzymologists?
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Explain down bellow what happens to the cell in pictures not in words:
Decreased pH in mitochondria
Increased ATP
Decreased pH in cytosol
Increased hydrolysis
Decreasing glycogen and triglycerides
Increased MAP kinase activity
Poor ion transport → For each one:→ What normally happens?→ What is wrong now?→ How does it mess up the cell?
1.) Community Diversity:
The brown and orange line represent two different plant communities.
a. Which color represents the community with a higher species richness?
b. Which color represents the community with a higher species evenness?
Relative abundance
0.1
0.04
0.001
2 4 6
8
10 12 14 16 18 20 22 24
Rank abundance
c. What is the maximum value of the Simpson's diversity index (remember, Simpson's
index is D = p², Simpson's diversity index is 1-D)?
d. If the Simpson's diversity index equals 1, what does that mean about the number of
species and their relative abundance within community being assessed?
1.) Community Diversity:
The brown and orange line represent two different plant communities.
a. Which color represents the community with a higher species richness?
b. Which color represents the community with a higher species evenness?
Relative abundance
0.1
0.04
0.001
2 4 6
8
10 12 14 16 18 20 22 24
Rank abundance
c. What is the maximum value of the Simpson's diversity index (remember, Simpson's
index is D = p², Simpson's diversity index is 1-D)?
d. If the Simpson's diversity index equals 1, what does that mean about the number of
species and their relative abundance within community being assessed?
Chapter 12 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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