Campbell Biology in Focus
Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 12.3, Problem 3CC

WHAT IF? Genes A, B, and C are located on the same chromosome. Testcrosses show that the recombination frequency between A and B is 28% and that between A and C is 12%. Can you determine the linear order of these genes? Explain.

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Linkage Mapping Using Dihybrid Testcrosses in Fruit Flies In fruit flies, the normal honey-gray body-color (bl*) is dominant to black (bl), having normal red eyes (pu+) is dominant to purple (pu), and having normal wings (vg+) is dominant to vestigial (vg). The three genes are located close together on the same chromosome. Your task is to use data from three dihybrid testcrosses to (1) calculate recombination frequencies, (2) convert recombination frequencies into map distances, and (3) build a map of the chromosome interval covered by the three genes, indicating their order and distances between them. BLACK PURPLE DIHYBRID CROSS In the parental generation, you mate a pure-breeding wild-type female (bl+/blt;put/pu+) with a pure-breeding black, purple male (bl/bl;pu/pu) to produce an F1 generation that is all wild-type (bl+/bl;pu*/pu). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (bl+/bl;pu*/pu) with tester males, which are black, purple…
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Campbell Biology in Focus

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