BIOLOGY
5th Edition
ISBN: 9781265202859
Author: BROOKER
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Chapter 12.2, Problem 1CS
Core Skill: Connections Look back at the role of DNA polymerase shown in Figure 11.15. What are similarities and differences between the function of DNA polymerase and that of RNA polymerase?
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Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research.
Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity.
The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c.
The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp).
Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…
4. Working backwards challenge
You have been hired as a genetic engineer. Your patient, Mr. Williams, is a 42-year-old male who has diabetes. To regulate his blood sugar
levels, Mr. Williams must test his blood daily and give himself insulin injections when necessary. Mr. Williams has contacted you to genetically
engineer bacteria to produce insulin. To genetically engineer bacteria, the section of DNA that codes for insulin is inserted into the bacterial
plasmid. You must work backwards to determine the DNA sequence.
Insulin consists of 51 amino acids. A small segment of the protein is provided below. Use the mRNA codon charts underneath to determine
the DNA sequence that would code for this segment of insulin.
Amino Acid
Start
Glycine
Leucine
Valine
Glutamic Acid
Proline
Stop
MRNA
AUG
AVAV
UGA
DNA
ТАС
Gv
TVTV
ACT
Second Base
P Type here to search
a
Solve 21
Chapter 12 Solutions
BIOLOGY
Ch. 12.1 - What disease would result if a person inherited...Ch. 12.1 - Prob. 2CCCh. 12.1 - What is the direction of flow of genetic...Ch. 12.2 - Core Skill: Connections Look back at the role of...Ch. 12.3 - Prob. 1CCCh. 12.4 - Prob. 1CCCh. 12.4 - Prob. 1EQCh. 12.4 - Prob. 2EQCh. 12.4 - Prob. 3EQCh. 12.5 - Core Skill: Connections Look back at Figure 6.3,...
Ch. 12.5 - Prob. 2CSCh. 12.6 - Prob. 1CCCh. 12 - Which of the following best represents the central...Ch. 12 - A mutation prevents a gene from being transcribed...Ch. 12 - Prob. 3TYCh. 12 - Prob. 4TYCh. 12 - If a eukaryotic mRNA failed to have a cap attached...Ch. 12 - Prob. 6TYCh. 12 - Prob. 7TYCh. 12 - During the initiation of translation, the first...Ch. 12 - Prob. 9TYCh. 12 - Prob. 10TYCh. 12 - Prob. 1CQCh. 12 - Prob. 2CQCh. 12 - Prob. 3CQCh. 12 - Prob. 1COQCh. 12 - Prob. 2COQ
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