PRIN. OF LIFE (LL)-W/ACHIEVE
3rd Edition
ISBN: 9781319492069
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 12.1, Problem 1FQ
Summary Introduction
To describe: That why PCR amplification of individual single-stranded –DNA produces half the DNA molecules in a cluster complementary and half identical to the original single-stranded molecule in high throughput DNA sequencing.
Introduction: The genome of an organism is defined as the whole genetic information that is inherited from one generation to the next generation. It includes the protein-coding genes, nonprotein coding genes, regulatory genes, chloroplast DNA and mitochondrial DNA. The human genome consists of about 3.1 billion genes of which only 20,000 genes are protein-coding genes.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 12 Solutions
PRIN. OF LIFE (LL)-W/ACHIEVE
Ch. 12.1 - Prob. 1LOCh. 12.1 - Prob. 2LOCh. 12.1 - Prob. 3LOCh. 12.1 - Prob. 4LOCh. 12.1 - Prob. 1FQCh. 12.1 - Prob. 2FQCh. 12.1 - Prob. 3FQCh. 12.1 - Prob. 1RACh. 12.1 - Prob. 2RACh. 12.1 - Prob. 3RA
Ch. 12.2 - Prob. 1LOCh. 12.2 - Prob. 2LOCh. 12.2 - Prob. 3LOCh. 12.2 - Prob. 4LOCh. 12.2 - Prob. 5LOCh. 12.2 - Prob. 1RACh. 12.2 - Prob. 2RACh. 12.2 - Prob. 3RACh. 12.2 - Prob. 4RACh. 12.2 - Prob. 1TKCh. 12.2 - Prob. 3TKCh. 12.3 - Prob. 1LOCh. 12.3 - Prob. 2LOCh. 12.3 - Prob. 3LOCh. 12.3 - Prob. 1RACh. 12.3 - Prob. 2RACh. 12.3 - Prob. 3RACh. 12.4 - Prob. 1LOCh. 12.4 - Prob. 2LOCh. 12.4 - Prob. 3LOCh. 12.4 - Prob. 4LOCh. 12.4 - Prob. 5LOCh. 12.4 - Prob. 1ICh. 12.4 - Prob. 2ICh. 12.4 - Prob. 3ICh. 12.4 - Prob. 1RACh. 12.4 - Prob. 2RACh. 12.4 - Prob. 3RACh. 12 - Prob. 1.1QCh. 12 - Prob. 1.2QCh. 12 - Prob. 1.3QCh. 12 - Prob. 2.1QCh. 12 - Prob. 2.2QCh. 12 - Prob. 2.3QCh. 12 - Prob. 3.1QCh. 12 - Prob. 3.2QCh. 12 - Prob. 3.3QCh. 12 - Prob. 4.1QCh. 12 - Prob. 4.2QCh. 12 - Prob. 4.3Q
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