The nucleotide sequence for the given region is to be proposed. Also, the sequence of the mutant oligonucleotide that would use for PCR amplification when the middle Ser residue is changed to a Cys residue is to be identified. Concept introduction: The technique that is used to amplify the fragments of the DNA template to form particular fragments of DNA in vitro is known as the polymerase chain reaction (PCR). The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.

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Chapter 12, Problem 8P

Interpretation Introduction

Interpretation:

The nucleotide sequence for the given region is to be proposed. Also, the sequence of the mutant oligonucleotide that would use for PCR amplification when the middle Ser residue is changed to a Cys residue is to be identified.

Concept introduction:

The technique that is used to amplify the fragments of the DNA template to form particular fragments of DNA in vitro is known as the polymerase chain reaction (PCR).

The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.

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