LooseLeaf for Human Anatomy
LooseLeaf for Human Anatomy
5th Edition
ISBN: 9781259285271
Author: Michael McKinley Dr., Valerie O'Loughlin, Elizabeth Pennefather-O'Brien
Publisher: McGraw-Hill Education
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Chapter 12, Problem 8MC
Summary Introduction

Introduction:

Eversion is the motion of the foot that occurs at transverse tarsal joints and subtalar joints. Eversion movement is helpful for walking, running, and stabilization of the body.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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