Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 12, Problem 2R
A mixed culture of Escherichia coli and Penicillium chrysogenum is inoculated onto the following culture media. On which medium would you expect each to grow? Why?
- a. 0.5% peptone in tap water
- b. 10% glucose in tap water
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Define what is a minimal medium and the nature of its components.
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply)
a - There is fungal growth in the original broth culture tube.
b- There is too much growth on the agar slant.
c- There are colonies of similar morphology on the slant.
d - There are red, yellow, and white colonies on the slant.
e - There is no growth on the slant.
For each of the following situations,
describe how bacteria on these growth.
media would appear on the plate:
A. On MacConkey agar, a bacterium that
grows on the medium but is negative for
lactose fermentation
B. On EMB agar, a bacterium that grows
on the medium but is positive for lactose
fermentation
C. On mannitol salt agar, a bacterium that
failed to grow on the medium
D. On mannitol salt agar, a bacterium that
grew on the medium and fermented
mannitol
Chapter 12 Solutions
Microbiology: An Introduction
Ch. 12 - Prob. 1RCh. 12 - A mixed culture of Escherichia coli and...Ch. 12 - Prob. 3RCh. 12 - Prob. 4RCh. 12 - Differentiate cellular and plasmodial slime molds....Ch. 12 - Prob. 6RCh. 12 - Prob. 7RCh. 12 - Prob. 8RCh. 12 - Prob. 9RCh. 12 - DRAW IT A generalized life cycle of the liver...
Ch. 12 - How many phyla are represented in the following...Ch. 12 - Prob. 2MCQCh. 12 - Prob. 3MCQCh. 12 - Fleas are the intermediate host for Dioylium...Ch. 12 - Prob. 5MCQCh. 12 - Prob. 6MCQCh. 12 - The definitive host for Plasmodium vivax is a....Ch. 12 - Prob. 8MCQCh. 12 - Prob. 9MCQCh. 12 - Prob. 10MCQCh. 12 - Alexandrium (red tide) has been called a plant...Ch. 12 - The life cycle of the fish tapeworm...Ch. 12 - Trypanosoma brucei gambiense-part (a) in the...Ch. 12 - Prob. 1CAECh. 12 - Prob. 2CAECh. 12 - A teenaged male in California complained of...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Plants use the process of photosynthesis to convert the energy in sunlight to chemical energy in the form of su...
Campbell Essential Biology with Physiology (5th Edition)
CAUTION How can evolutionary fitness be estimated? a. Document how long individuals survive. b. Count the numbe...
Biological Science (6th Edition)
Your bore cells, muscle cells, and skin cells look different because a. different kinds of genes are present in...
Campbell Essential Biology (7th Edition)
5.4 Genes E and H are syntenic in an experimental organism with the genotype . Assume
that during each meiosis,...
Genetic Analysis: An Integrated Approach (3rd Edition)
Why are BSL-4 suits pressurized? Why not just wear tough regular suits?
Microbiology with Diseases by Body System (5th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths? 2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate? 3) What are the advantages and disadvantages of streak plating compared with pour plating?arrow_forwardA. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?arrow_forwardA culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.arrow_forward
- Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three different media, namely I, II, and III, and the growth of each bacterial strain has been followed by spectrophotometry (Figure 1). Strain A Strain B Strain C 4.5 45 Medium I 4 3.5 25 2.5 3 E 2.5 Minimal medium + Glucose 25 2.5 O 15 1 0.5 as 05 100 200 300 400 500 600 100 200 400 600 200 Time (minj Strain A Strain B StrainB 4.5 4s 4s Medium II 3.5 15 25 2 3 Minimal 25 2 medium + Glucose 1 + Arginine 0.5 as as 100 200 300 400 500 600 100 300 400 s00 600 100 300 400 600 Tme Time (min) Strain A me StreinB Streine 4.5 45 45 Medium II 4 4. 3.5 3.5 25 2 3 Minimal medium + Glucose 5 2.5 25 25 8 15 15 + Lactose 1 0.5 05 05 100 200 300 400 500 600 300 200 400 10 200 300 630 Time (min) Tine imin Time min Figure 1. Growth curves of the three E. coli strains A, B and C in three media: I, minimal medium containing glucose; II, minimal medium containing glucose and arginine; and III, minimal medium…arrow_forwardBased on the following image, which concentration of antibiotic would be considered the minimum bactericidal concentration (MBC)? a. 8 ug/mlb. 16 ug/mlc. 25 ug/mlarrow_forwardBelow is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.arrow_forward
- Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.arrow_forwardA culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ularrow_forwardYou spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.arrow_forward
- You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria.arrow_forward3b)A plant suspension culture was used to study the effect of three types of media on the growth of the culture using batch culture approach. Figure 3.1 shows the growth of the plant suspension cultures using Murashige and Skoog (MS), Gamborg B5 and Vacin and Went media. Comment and conclude on the results obtained. Suggest which is the most suitable medium to maintain the suspension cultures with justification.arrow_forwardAnswer the attached photo belowarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Microbial Nutrition and Growth; Author: Scientist Cindy;https://www.youtube.com/watch?v=rK3UkyWjkl8;License: Standard YouTube License, CC-BY