Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 12, Problem 2CT
Fill in the blanks in this diagram to make a key to these bacteria:
- Eschenchia coil
- Staphylococcus aureus
- Alcaligenes faecalis
- Staphylococcus epidermidis
Circle or highlight the gram-positive bacteria in one color; use a different color to circle or highlight the gram-negative bacteria.
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In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT:
The primary stain, crystal violet, is dark purple
The counterstain (safranin) causes the purple color to develop
Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol
Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added
None of the other four answers (All are reasons that gram-positive bacteria are purple
This bacteria is commonly found in soil and water, and can cause otitis externa (aka swimmers ear).
Pseudomonas aeruginosa
Streptococcus pyogenes
Staphylococcus aureus
Staphylococcus epidermidis
Which one of the following statements about the Gram stain is false?
A-In the Gram stain, a dye called crystal violet is used to stain the cells purple.
B-Gram positive cells end up being stained purple.
C-Gram negative cells have thinner peptidoglycan layers than Gram positive bacteria.
D-In the Gram stain, alcohol is used to kill the bacteria
E-Gram negative cells end up being stained pink or red.
Chapter 12 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- describe the shape of the bacterial cells for each of the two yogurt bacteria. Be sure to use the terms for bacteria shapes that you learned in the Survey of the Microbial World lab!arrow_forwardPut the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersionarrow_forwardA Gram stain indicates purple cells that are round and attached in chains. Which of the following best describes these results? A Gram stain indicates purple cells that are round and attached in chains. Which of the following best describes these results? Gram-negative streptococci Gram-negative streptobacilli Gram-positive streptococci Gram-positive staphylococci Gram-positive cocci, sarcinaearrow_forward
- Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient Agararrow_forwardMycoplasmas are pleomorphic bacteria. How do the unique structural characteristics of mycoplasmas relate to their bacterial morphology? Mycoplasmas have a low G + C genome content. Mycoplasmas appear Gram negative when a Gram stain is performed. Mycoplasmas lack a cell wall. Mycoplasmas form colonies with a fried-egg appearance.arrow_forwardWhat color would Staphylococcus aureus cells be with the endospore stain shown in the photo?arrow_forward
- characterize the bacteriaarrow_forwardAll of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteinsarrow_forwardWhich type of hemolysis characterizes Streptococcus pyogenes, the bacterium that causes strep throat?arrow_forward
- What specifically causes colonies of lactose fermenters to be pink-red on MacConkey agar?arrow_forwardA farmer working on a piece of machinery gets his shirtsleeve caught in a moving piece of the equipment. His shirt is sliced, and a sharp blade covered in mud slices through his upper arm. He attempts to control the bleeding and immediately seeks medical attention. After 3 days, he develops a fever and his arm becomes extremely swollen and painful. Pulling back the bandages, he finds that the wound has become blackened and is leaking bloody fluid. Microscopic analysis of the fluid reveals the presence of gram-positive bacilli. a. Discuss what condition the patient is suffering from and the likely causative agent of this infection. b. Explain how the patient contracted this pathogenic microbe and what virulence factors contributed to the pathogenesis seen at the wound site. c. In addition to antibiotics, the physician prescribes hyperbaric therapy. Describe what this treatment involves and how it could be therapeutic to this patient.arrow_forwardWhy is the fact that Pseudomonas species can grow in nutrient-poorenvironments medically important?arrow_forward
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