Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
4th Edition
ISBN: 9781259853197
Author: Robert Brooker
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 12, Problem 2COQ
Summary Introduction

To determine: The list of similarities and difference in the events of initiation, elongation, and termination stages of translation.

Introduction: The gene expression is a highly complex but coordinated process. The information present in the DNA is transferred to RNA molecule by the process called transcription in which dsDNA provides a template for the synthesis of the RNA molecule. The translation, on the other hand, is the passing of the information transferred from mRNA to make a corresponding peptide chain according to the information coded in the mRNA molecule.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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