Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them. a. Find the done with the gene for lactase. b. Insert the plasmids into bacteria and grow the bacteria into clones. c. Isolate the gene for lactase. d. Create recombinant plasmids, including one that carries the gene for lactase.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Svp je voulais demander l aide pour mon exercice

Imagine that you are a clinical geneticist. Your colleague is an oncologist who wants your help explaining the basics of genetics to their patient, who will be undergoing genetic testing in the coming weeks for possible acute myeloid leukemia (AML) induced by the radiation she had several years ago for breast cancer. Write a 1,050- to 1,225-word memo to your colleague. Include the following in your memo: An explanation of the molecular structure of DNA and RNA, highlighting both similarities and differences A description of the processes of transcription and translation An explanation of the differences between leading and lagging strands and how the DNA is replicated in each strand Reponses to the following common questions patients might ask about this type of genetic testing and genetic disorder: Does AML run in families? What genes are tested for?

Respond to the following in a minimum of 175 words: What are some potential consequences that could result if the processes of replication, transcription, and translation don’t function correctly? Provide an example of how you might explain these consequences in terms that patients might understand.

Answer 2

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 8

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer 12

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Answer 13

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 14

Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - Prob. 3SQ Ch. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - What feature of a DNA fragment causes it to move...Ch. 12 - After a gel electrophoresis procedure is run, the...Ch. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Put the following steps of human gene therapy in...Ch. 12 - For each statement, identify which major theme is...

Concept explainers

Videos

Answer to Problem 1SQ

Explanation of Solution

Want to see more full solutions like this?

Chapter 12 Solutions