Chapter 12, Problem 3SQ

Summary Introduction

To determine:

The benefit of using restriction enzyme that cuts the DNA in staggered fashion in making recombinant DNA.

Introduction:

Restriction enzyme are the endonucleases which cuts the double stranded DNA after recognizing specific short sequence of DNA called recognition sequence and cleave the both strand of DNA at or near the recognition site. Restriction enzymes cut the DNA and produce two types of fragments, DNA fragment with blunt end and DNA fragment with cohesive end.