To propose: A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat. Introduction: Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

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Answer 1

Question

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

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Draw a diagram to demonstrate 3' to 5' exonuclease activity?

1. Write the piece of mRNA that would code for the amino acid sequence NH3 - met - tyr - cys- his- CO2. Label the 5' and 3' ends. (diagram attached) 2.

You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

Answer 2

Question

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Expert Solution & Answer

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Answer 3

Question

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Expert Solution & Answer

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See solution

Answer 4

Question

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Expert Solution & Answer

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Answer 5

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Answer 6

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

Answer 7

Chapter 12, Problem 18P

(a)

Summary Introduction

To propose:

A reasonable model to explain that exposing a sensory neuron containing TRPV1 to high temperature leads to a sensation of heat.

Introduction:

Neuron is also known as nerve cell is defines as the cell that carries electrical impulses. It serves as the basic unit of nervous system. It is composed of axon, cell body and dendrites.

Answer 8

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

Answer 9

(b)

Summary Introduction

To explain:

The few drops of hot peper sauce can taste very hot without actually burning you.

Introduction:

Capsaicin is defined as an active compound of chili peppers produced by plants of genus Capsicum. It is irritant for mammals including humans as it produces a burning sensation in tissues when it comes in contact.

Answer 10

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Answer 11

(c)

Summary Introduction

To determine:

The sensations produced from contact with low and high levels of menthol.

Introduction:

Menthol is an organic compound which is obtained from corn mint, peppermint or other mint oils. TRPM8 is a transient receptor potential cation channel belongs to M member 8 is a type of proteins which is encoded by TRPM8 gene. It is molecular transducer of cold somatosensation in humans.

Answer 12

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Answer 15

Ch. 12 - Prob. 1P Ch. 12 - Prob. 2P Ch. 12 - Prob. 3P Ch. 12 - Prob. 4P Ch. 12 - Prob. 5P Ch. 12 - Prob. 6P Ch. 12 - Prob. 7P Ch. 12 - Prob. 8P Ch. 12 - Prob. 9P Ch. 12 - Prob. 10P

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