The making of a reporter gene construct using the n o x promoter is to be described. Also, the validation of the fact that the yeast strain bearing this reporter gene construct might be an effective oxygen sensor is to be stated. Concept introduction: A reporter gene refers to a gene that is attached by the researchers to a nucleic acid segment that can increase or decrease the expression of specific genes in an organism. Reporter genes can also report on various properties like the strength of promoters.

Question

Want to see the full answer?

Answer 1

Question

Chapter 12, Problem 14P

Interpretation Introduction

Interpretation:

The making of a reporter gene construct using thenoxpromoter is to be described. Also, the validation of the fact that the yeast strain bearing this reporter gene construct might be an effective oxygen sensor is to be stated.

Concept introduction:

A reporter gene refers to a gene that is attached by the researchers to a nucleic acid segment that can increase or decrease the expression of specific genes in an organism. Reporter genes can also report on various properties like the strength of promoters.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2

what are the different classes and some examples of neuroprotectants that can be used to treat, prevent, or combat neurotoxicity/a neurotoxicant...for example, antioxidants, nutraceuticals, etc.,..?

Imagine that aldolase can react with the seven carbon molecule Sedoheptulose-1,7-bisphosphate (below). Use the mechanism to predict the two products generated. Please draw out the stereochemistry in a fischer projection.