Microbiology with Diseases by Taxonomy (5th Edition)
Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 11, Problem 9MC
Summary Introduction

Introduction:

Nitrogen is one of the most essential macromolecules needed by plants for their proper functioning. Nitrogen deficiency results in slow and stunted growth. There are some other soil organisms like Cyanobacteria which are able to convert molecular nitrogen into ammonium. Plants also use an ammonium form of nitrogen to increase their growth.

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From your previous experiment, you found that this enhancer activates stripe 2 of eve expression. When you sequence this enhancer you find several binding sites for the gap gene, Giant. To test how Giant interacts with eve, you decide to remove all of the Giant binding sites from the eve enhancer. What results do you expect to see with respect to eve expression?
What experiment could you do to see if the maternal gene, bicoid, is sufficient to form anterior fates?
You’re curious about the effect that gap genes have on the pair-rule gene, evenskipped (eve), so you isolate and sequence each of the eve enhancers. You’re particularly interested in one of the enhancers, which is just upstream of the eve gene.  Describe an experimental technique you would use to find out where this particular eve enhancer is active.
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