MICROBIOLOGY-ACCESS >CUSTOM<
MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Chapter 11, Problem 4R
Summary Introduction

Introduction

Methanogens belong to the archaea domain and are obligate anaerobes. They are extremophiles and they live in extreme environmental conditions, like high temperatures. The cell wall present in this domain helps in its protection. They are syntrophic in nature.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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