Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 11, Problem 3CT
Will the isolated colonies always be in the fourth sector on the streak plate?
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Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?arrow_forwardWhat is the purpose of a streak plate and how do you know one was successful? Explain.arrow_forwardWhy is it necessary to sterilize the loop between streaks when streaking for single colonies?arrow_forward
- Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 1 x 10 fungal CFU/mL (or CFU/g) the entire lot produced will be rejected. Kousei's boss, Kaori, wanted to know if the lot passed the FDA guidelines. The following fungal counts were obtained after serial dilution and spread plating of the samples: Type of Dilutions plated 10-4 10-3 105 Colony Plate 1 Plate 2 Plate 1 Plate 2 Plate 1 Plate 2 Plate 3 166 Fungi 160 155 45 40 1 11 Based on the fungal counts obtained, will the lot be rejected? No, because the computed CFU/mL is 4.3 x 10^5 No, because the computed CFU/mL is 4.1 x 10^5 Yes, because the computed CFU/mL is 4.3 x 10^6 Yes, because the computed CFU/mL is 4.1 x 10^6 Yes, because the computed CFU/mL is 1.1 X 10^7 Information is lacking so CFU/mL cannot be computed Plate 3 37 Plate 3 7arrow_forwardLabel name asap quickly onlyarrow_forwardWhen bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?arrow_forward
- Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?arrow_forwardWhat was the purpose of leaving the cover on one set of agar plates? How did the growth on these plates differ from the growth on the uncovered plates that were exposed to UV light for the same amount of time?arrow_forwardYou inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?arrow_forward
- What do you think the best method to detect Toxoplasma infection ?? Write the short introduction and the method??arrow_forwardWhat is the purpose of a streak plate? Name another means of achieving the same purpose?arrow_forwardHow is it that mold appeared on my samples? (in other words where did it come from or what is the source of the mold that grows on the samples?) I had 10 bags each bag had 3 slices of apple and 3 pieces of bread, 5 bags where left in a dark warm place and 5 where left in a bright warm place. 2 bags were control bags, 2 contained the fruit/bread and 1 tsp of vinegar, 2 contained fruit/bread and 1 tsp of water, 2 contained fruit/bread and 1 tsp of lemon juice, and 2 contained fruit/bread and 1 tsp of sugar water. The fruit and bread were mixed together in the same bag.arrow_forward
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