LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version
LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version
14th Edition
ISBN: 9781259705236
Author: Alfred E Brown Ph.D., Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 11, Problem 1SAQ
Summary Introduction

To explain: The chromophores.

Introduction: The staining method of bacteria with one stain alone is called simple staining. Usually, acidic stains and basic stains are used to stain bacteria. The simple staining techniques are used to identify the morphological characteristics along with the size and arrangement of bacteria such as a spherical shape, rod shape, spiral shape, comma shape, and so on.

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A stain is the organic compound that consists of an auxochrome group and chromophore group. A chromophore is a color-imparting compound that binds to the charged cellular components such as proteins, nucleic acids, and so on. Acidic stain imparts a negative charge on the chromophore after dissociation; and hence, it binds to the positive charge of the bacterial cell, whereas basic stain imparts positive charge on the chromophore after dissociation; and hence, it binds to the negative charge of the bacterial cell.

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