Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 11, Problem 1CT
Can some bacteria grow on the streak plate and not be seen if the pour plate technique is used? Explain.
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Laboratory Experiments in Microbiology (11th Edition)
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?arrow_forwardDESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.arrow_forwardWhat is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?arrow_forward
- What is the difference between streak plate method and spread plate technique?arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forwardIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forward
- Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?arrow_forwardWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handlearrow_forwardif an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?arrow_forward
- Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the microorganisms came from?arrow_forwardWhat is the best streaking technique in a culture media? Explainarrow_forwardWhat stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forward
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