The reason for inactivation of X chromosome in the mammalian females. Introduction: Mammals are the vertebrate having mammary glands as one of their main characteristics which differentiate mammals from other groups. Mammalian females have developed mammary glands which are essential for the nourishment of the baby.

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Chapter 10.7, Problem 3MC

Summary Introduction

To determine:

The reason for inactivation of X chromosome in the mammalian females.

Introduction:

Mammals are the vertebrate having mammary glands as one of their main characteristics which differentiate mammals from other groups. Mammalian females have developed mammary glands which are essential for the nourishment of the baby.

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You have a bacterial strain with the CMU operon: a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a kinase and regulator corresponding to a two-component system. The cmu operon is activated when Pep 1 is added to the growth media. Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon. Pep1 cmu-kinase cmu-regulator You also have these genetic components in other strains: b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a series of multiple operons that together encode a transformasome (cellular machinery for transformation). c) the gene cl (a repressor). d) the promoter X, which includes a cl binding site (and in the absence of cl is active). e) the gene gp (encoding a green fluorescence protein). Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange any of these genomic features, how would you use one or more of these to modify the…