Genetics: From Genes To Genomes (6th International Edition)
6th Edition
ISBN: 9781260041217
Author: Leland Hartwell Dr., ? Michael L. Goldberg Professor Dr., ? Janice Fischer, ? Leroy Hood Dr.
Publisher: Mcgraw-Hill
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Textbook Question
Chapter 10, Problem 7P
Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas geneticists studying bacteria almost never do? Why might bacterial geneticists have difficulties constructing cDNA libraries even if they wanted to?
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What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned
a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid
carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence
of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his
gene of interest using the bioinformatics databases available.
What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria? How will you identify clones of interest?
Chapter 10 Solutions
Genetics: From Genes To Genomes (6th International Edition)
Ch. 10 - Prob. 1PCh. 10 - List three independent techniques you could use to...Ch. 10 - Figure 10.2a has numbers indicating the...Ch. 10 - Which of the enzymes from the following list would...Ch. 10 - Prob. 5PCh. 10 - a. What sequence information about a gene is...Ch. 10 - Why do geneticists studying eukaryotic organisms...Ch. 10 - Consider three different kinds of human libraries:...Ch. 10 - The human genome has been sequenced, but we still...Ch. 10 - This problem investigates issues encountered in...
Ch. 10 - For the sake of simplicity, Fig. 10.4 omitted one...Ch. 10 - Give two different reasons for the much higher...Ch. 10 - Using a cDNA library, you isolated two different...Ch. 10 - The figure that follows shows part of a modified...Ch. 10 - In Problem 14, cDNAs F and G could not be found in...Ch. 10 - Fig. 10.10 presents a model for exon shuffling in...Ch. 10 - An interesting phenomenon found in vertebrate DNA...Ch. 10 - a. If you found a zinc-finger domain which...Ch. 10 - Prob. 19PCh. 10 - In the human immune system, so-called B cells can...Ch. 10 - Chimpanzees have a set of hemoglobin genes very...Ch. 10 - Complete genome sequences indicate that the human...Ch. 10 - On your computers browser, view the page accessed...Ch. 10 - Prob. 24PCh. 10 - Prob. 25PCh. 10 - Certain individuals with mild forms of...Ch. 10 - The 1 and 2 genes in humans are identical in their...Ch. 10 - Prob. 28P
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is an advantage of making a cDNA library rather than a genomic library?arrow_forwardIhsan is a biologist working with the genetics of a psychrophilic bacterium. He cloned an antifreeze gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Ihsan finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardIf you made a cDNA library from human brain cells and one from human liver cells would the clones in your two libraries be the same? a)Yes, the libraries would be identical. b)You cannot make cDNA libraries from these cell types. c) No, the libraries would have few overlapping clones. d) The libraries would have a number of similar clones for common cellular proteins but would have a number of unique clones associated specifically with each cell type.arrow_forward
- What is the difference between a genomic library and a cDNA library?arrow_forwardApart from genome size, what factors make completeassembly of a eukaryotic genome more difficult than assemblyof a genome from a species of Bacteria or Archaea?arrow_forwardThe idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)arrow_forward
- Although a number of different animals have been successfully cloned, the process of creating cloned animals is very inefficient. Typically, only about 0.1% to 3% of attempts result in a live-born animal. What might be some of the reasons for this low rate of success?arrow_forwardIn 1995, the first free-living organism to have its genome completely sequenced was Haemophilus influenzae, a bacteria. In the following year, the baker’s yeast Saccharomyces cerevisiae was the first eukaryote genome sequence to be fully sequenced. The complete sequencing of the human genome and related organisms represent one of the greatest scientific achievements in the history of mankind.Elaborate on the importance of genome studies in general.arrow_forwardIn the practical you have been analysing a human genomic library. You know from your calculations that only a small proportion of the human genome is represented, even when the entire class results are considered. Therefore, the chance of finding a particular single-copy gene in your library is very small. Outline a strategy for constructing a genomic DNA library more representative of the entire human genome. You will need to consider alternative vectors and the efficiency of transformation of the bacterial cells.arrow_forward
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