BIOCHEMISTRY (LL)
9th Edition
ISBN: 9781337805100
Author: Campbell
Publisher: CENGAGE L
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Chapter 10, Problem 51RE
RECALL How does
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Example 1:
1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the
kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the
enzyme?
A) 1050 µM/s.
B) 100 µM/s.
C) 150 μM/s.
D) 105 μM/s.
In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat
liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment,
incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled
lecithin. This became their assay for the enzymes involved in lecithin synthesis.
Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values.
-O-P-O-CH2-CH₁₂-N(CH3)3
Phosphocholine
H₂C-O-C-R
HC-O-C-R2
+
+
+
Cell fraction + ?
HC-O-P-O-CH₁₂-CH₂-N(CH),
O
Phosphatidylcholine
The researchers then centrifuged the broken cell preparation
to separate the membranes from the soluble proteins. They
tested three preparations: whole extract, membranes, and
soluble proteins. Table 1 summarizes the results.
Table 1: Cell fraction requirement for incorporation of
32p-phosphocholine into…
Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following
observations and experiments.
(a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized
and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and
53.29% O by weight.
(b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u).
(c) Infrared spectroscopy showed that X contained one double bond.
(d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter.
(e) The aqueous solution of X is acidic.
What is the empirical formula of X?
Chapter 10 Solutions
BIOCHEMISTRY (LL)
Ch. 10 - RECALL Define replication, transcription, and...Ch. 10 - REFLECT AND APPLY Is the following statement true...Ch. 10 - REFLECT AND APPLY Why is it more important for DNA...Ch. 10 - RECALL Why is the replication of DNA referred to...Ch. 10 - RECALL What is a replication fork? Why is it...Ch. 10 - Prob. 6RECh. 10 - RECALL Why is it necessary to unwind the DNA helix...Ch. 10 - REFLECT AND APPLY In the MeselsonStahl experiment...Ch. 10 - REFLECT AND APPLY Suggest a reason why it would be...Ch. 10 - RECALL Do DNA-polymerase enzymes also function as...
Ch. 10 - RECALL Compare and contrast the properties of the...Ch. 10 - REFLECT AND APPLY Define processivity, and...Ch. 10 - REFLECT AND APPLY Comment on the dual role of the...Ch. 10 - REFLECT AND APPLY What is the importance of...Ch. 10 - REFLECT AND APPLY DNA synthesis always takes place...Ch. 10 - REFLECT AND APPLY What would happen to the...Ch. 10 - Prob. 17RECh. 10 - REFLECT AND APPLY Why is it not surprising that...Ch. 10 - Prob. 19RECh. 10 - RECALL List the substances required for...Ch. 10 - RECALL Describe the discontinuous synthesis of the...Ch. 10 - RECALL What are the functions of the gyrase,...Ch. 10 - RECALL Single-stranded regions of DNA are attacked...Ch. 10 - RECALL Describe the role of DNA ligase in the...Ch. 10 - RECALL What is the primer in DNA replication?Ch. 10 - Prob. 26RECh. 10 - REFLECT AND APPLY Why is a short RNA primer needed...Ch. 10 - Prob. 28RECh. 10 - RECALL What was the recent change in the estimated...Ch. 10 - Prob. 30RECh. 10 - RECALL Why is a clamp loader necessary in...Ch. 10 - RECALL How does proofreading take place in the...Ch. 10 - Prob. 33RECh. 10 - Prob. 34RECh. 10 - BIOCHEMICAL CONNECTIONS Of what benefit is it for...Ch. 10 - REFLECT AND APPLY Your book contains about 2...Ch. 10 - REFLECT AND APPLY E. coli incorporates...Ch. 10 - REFLECT AND APPLY Given the typing speed from...Ch. 10 - Prob. 39RECh. 10 - REFLECT AND APPLY How can breakdown in DNA repair...Ch. 10 - Prob. 41RECh. 10 - RECALL What is a direct way of repairing...Ch. 10 - Prob. 43RECh. 10 - Prob. 44RECh. 10 - Prob. 45RECh. 10 - Prob. 46RECh. 10 - RECALL How did Messelson and Weigle demonstrate...Ch. 10 - Prob. 48RECh. 10 - RECALL What is the Holliday Model?Ch. 10 - RECALL Do eukaryotes have fewer origins of...Ch. 10 - RECALL How does DNA replication in eukaryotes...Ch. 10 - Prob. 52RECh. 10 - REFLECT AND APPLY (a) Eukaryotic DNA replication...Ch. 10 - Prob. 54RECh. 10 - Prob. 55RECh. 10 - Prob. 56RECh. 10 - Prob. 57RECh. 10 - Prob. 58RECh. 10 - Prob. 59RECh. 10 - Prob. 60RECh. 10 - Prob. 61RECh. 10 - Prob. 62RECh. 10 - Prob. 63RECh. 10 - Prob. 64RE
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