Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 10, Problem 2A
Describe the use of a DNA probe and PCR for:
- a. rapid identification of an unknown bacterium.
- b. determining which of a group of bacteria are most closely related.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Describe the use of a DNA probe and Pcr. A. for rapid identification of an unknown bacterium.
B determining which of a group of bacteria are most closely related.
Recombinant DNA techniques are used to custom –build bacteria for two main reasons: to make a multiple copies of desired genes and to obtain useful proteins. Give an example of each of these applications in the field of pharmaceutical, medicine and agriculture.
describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.
Chapter 10 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 10 - Which of the following organisms are most closely...Ch. 10 - Here is some additional information on the...Ch. 10 - DRAW IT Use the additional information below to...Ch. 10 - DRAW IT Use the information in the table below to...Ch. 10 - NAME IT Use the key in the Applications of...Ch. 10 - Sergeys Manual of Systematic Bactenoiogy differs...Ch. 10 - Bacillus and Lactobacillus are not in the same...Ch. 10 - Prob. 3MCQCh. 10 - Which of the following is false about scientific...Ch. 10 - You could identify an unknown bacterium by all of...
Ch. 10 - The wall-less mycoplasmas are considered to be...Ch. 10 - Use the following choices to answer questions 7...Ch. 10 - Prob. 8MCQCh. 10 - Prob. 9MCQCh. 10 - Use the following choices to answer questions 9...Ch. 10 - The GC content of Micrococcus is 66-75 moles %,...Ch. 10 - Describe the use of a DNA probe and PCR for: a....Ch. 10 - SF medium is a selective medium, developed in the...Ch. 10 - Prob. 1CAECh. 10 - Prob. 2CAECh. 10 - Prob. 3CAE
Additional Science Textbook Solutions
Find more solutions based on key concepts
On what molecule does the anticodon appear? Explain the role of this molecule in protein synthesis.
Human Physiology: An Integrated Approach (8th Edition)
Single penny tossed 20 times and counting heads and tails: Probability (prediction): _______/20 heads ________/...
Laboratory Manual For Human Anatomy & Physiology
How does the removal of hydrogen atoms from nutrient molecules result in a loss of energy from the nutrient mol...
SEELEY'S ANATOMY+PHYSIOLOGY
The validity of a scientific law.
Physical Universe
An obese 55-year-old woman consults her physician about minor chest pains during exercise. Explain the physicia...
Biology: Life on Earth with Physiology (11th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe what is produced by a PCR assay, how it is detected, and explain how it can determine whether a specific pathogen is present in a sample.arrow_forwardDetermining the genetic profile of a bacterium for its identification is a part of which step of the identification process?arrow_forwardDescribe the challenges of mono- versus multiplex PCR for detection of bacterial sequences in the environmentarrow_forward
- Order the following technical steps that are required to identify a bacterium by PCR and sequencing of the 16S rRNA gene. [1 mark] - Carry out thermal cycling (amplification) of the PCR - Checking the PCR product (amplication) by agarose gel electrophoresis - Purify the PCR product - Perform a DNA extraction - Sequence the PCR product(Sanger sequencing) - Grow a fresh overnigh liquid culture - Obtain a pure bacterial culture - Set up a 16S Rrna gene PCR - Perform bioinformatic analysis (BLAST)arrow_forwardecombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of diseases. Probes are now used for identification of what infectious agents ?arrow_forwardExplain how PCR can be used to detect infectious agents in diagnoses of diseases.arrow_forward
- 1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.arrow_forwardBased on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which antibiotic? Write the letter of the antibiotic below. G. B. E.arrow_forwardan established technique to measure gene expression in bacteria uses: A. A plasmid B. Bright-field microscopy C. Atomic force microscopy D. Gram staining E. Ethanolarrow_forward
- Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.arrow_forwardWhich of the following is not associated with gene therapy SCID patients A) utilizes an enzyme to repair the patient’s nonfunctioning ADA gene B) isolation of patient cells C) cloning of functioning a D a gene in bacteria D) use of vectorarrow_forwardnotes are attached below for further information Why is it important to incubate plates upside down (inverted)?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License