Essential Cell Biology (Fourth Edition)
Essential Cell Biology (Fourth Edition)
4th Edition
ISBN: 9780815345251
Author: Bruce Alberts, Dennis Bray, Karen Hopkin, Alexander D. Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
Publisher: W. W. Norton & Company
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Chapter 10, Problem 16Q
Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…
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