Campbell Essential Biology (7th Edition)
Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 10, Problem 15PS

A cell containing a single chromosome is placed in a median containing radioactive phosphate, making any new DNA strands formed by DNA replication radioactive. The cell replicates its DNA and divides. Then the daughter cells (still in the radioactive medium) replicate their DNA and divide, resulting in a total of four cells. Sketch the DNA molecules in all four cells, showing a normal (nonradioactive) DNA strand as a solid line and a radioactive DNA strand as a dashed line.

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Name something that could be happening to glutamate transporters that could have this effect on glutamate transport (i.e. the difference in glutamate transporters between control and experimental conditions). (Bonus: if you can also guess what the experimental condition is).
a. For the control condition, calculate Kd. Include units and show your work. b. For the control condition, calculate Bmax. Include units and show your work.  c. For the experimental condition, calculate Kd. Include units and show your work. For the experimental condition, calculate Bmax. Include units and show your work.
Now draw a rough sketch of what the control data might look like if in addition to the specific binding, there was also a considerable amount of nonspecific binding (again using a normal dose/response curve)
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