Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 10, Problem 10.4.3AYLO
Summary Introduction
To analyze:
The events of excitation-contraction coupling of muscle fibers and the roles of the sarcoplasmic reticulum, calcium, troponin and tropomyosin in excitation-contraction coupling.
Introduction:
The excitation-contraction coupling is the rapid communication between electrical events occurring in the plasma membrane of skeletal muscle fibers and
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 10 Solutions
Human Anatomy
Ch. 10.1 - What general function of muscular tissue...Ch. 10.1 - What are the basic structural differences between...Ch. 10.1 - Prob. 3BYGOCh. 10.1 - State five special properties of muscular tissue...Ch. 10.2 - Prob. 1AWYKCh. 10.2 - Prob. 5BYGOCh. 10.2 - Prob. 6BYGOCh. 10.2 - Answer the following questions to test your...Ch. 10.2 - Answer the following questions to test your...Ch. 10.2 - Answer the following questions to test your...
Ch. 10.2 - Prob. 10BYGOCh. 10.2 - Answer the following questions to test your...Ch. 10.3 - Prob. 12BYGOCh. 10.3 - Prob. 13BYGOCh. 10.3 - Prob. 14BYGOCh. 10.3 - Answer the following questions to test your...Ch. 10.3 - Prob. 16BYGOCh. 10.3 - Prob. 17BYGOCh. 10.4 - Prob. 1AWYKCh. 10.4 - What role does the sarcoplasmic reticulum play in...Ch. 10.4 - Prob. 19BYGOCh. 10.4 - Prob. 20BYGOCh. 10.4 - Answer the following questions to test your...Ch. 10.4 - Prob. 22BYGOCh. 10.5 - Prob. 23BYGOCh. 10.5 - Prob. 24BYGOCh. 10.5 - Prob. 25BYGOCh. 10.5 - Prob. 26BYGOCh. 10.6 - Answer the following questions to test your...Ch. 10.6 - What is the principal difference between the way...Ch. 10.6 - Prob. 29BYGOCh. 10.6 - Prob. 30BYGOCh. 10.6 - Prob. 31BYGOCh. 10 - The scope of myology and of the term muscular...Ch. 10 - Differences between skeletal, cardiac, and smooth...Ch. 10 - Muscle Types and Functions (p. 236) The multiple...Ch. 10 - Five physiological properties that muscle cells...Ch. 10 - General Anatomy of Muscles (p. 237) The tissues...Ch. 10 - General Anatomy of Muscles (p. 237) The...Ch. 10 - General Anatomy of Muscles (p. 237) The separation...Ch. 10 - General Anatomy of Muscles (p. 237) Orientation of...Ch. 10 - General Anatomy of Muscles (p. 237) The difference...Ch. 10 - General Anatomy of Muscles (p. 237) How an...Ch. 10 - General Anatomy of Muscles (p. 237) The...Ch. 10 - General Anatomy of Muscles (p. 237) Some muscle...Ch. 10 - General Anatomy of Muscles (p. 237) The meaning of...Ch. 10 - General Anatomy of Muscles (p. 237) The functional...Ch. 10 - General Anatomy of Muscles (p. 237) The...Ch. 10 - General Anatomy of Muscles (p. 237) The...Ch. 10 - General Anatomy of Muscles (p. 237) How to...Ch. 10 - Prob. 10.2.14AYLOCh. 10 - The internal ultrastructure of a skeletal muscle...Ch. 10 - The relationship between myofilaments, myofibrils,...Ch. 10 - Prob. 10.3.3AYLOCh. 10 - Prob. 10.3.4AYLOCh. 10 - Prob. 10.3.5AYLOCh. 10 - Prob. 10.3.6AYLOCh. 10 - Prob. 10.3.7AYLOCh. 10 - Prob. 10.3.8AYLOCh. 10 - Prob. 10.3.9AYLOCh. 10 - The structure of a neuromuscular junction and the...Ch. 10 - Prob. 10.3.11AYLOCh. 10 - The components of a motor unit; what is meant by...Ch. 10 - Prob. 10.3.13AYLOCh. 10 - Prob. 10.4.1AYLOCh. 10 - Prob. 10.4.2AYLOCh. 10 - Prob. 10.4.3AYLOCh. 10 - Prob. 10.4.4AYLOCh. 10 - Prob. 10.4.5AYLOCh. 10 - Prob. 10.4.6AYLOCh. 10 - Prob. 10.4.7AYLOCh. 10 - Prob. 10.4.8AYLOCh. 10 - Cardiac and Smooth Muscle (p. 254) The structure...Ch. 10 - Prob. 10.5.2AYLOCh. 10 - Prob. 10.5.3AYLOCh. 10 - Prob. 10.5.4AYLOCh. 10 - Prob. 10.5.5AYLOCh. 10 - Prob. 10.5.6AYLOCh. 10 - Prob. 10.6.1AYLOCh. 10 - Prob. 10.6.2AYLOCh. 10 - Prob. 10.6.3AYLOCh. 10 - The mode of inheritance and pathology of muscular...Ch. 10 - Prob. 10.6.5AYLOCh. 10 - Prob. 1TYRCh. 10 - Muscle cells must have all of the following...Ch. 10 - Prob. 3TYRCh. 10 - Prob. 4TYRCh. 10 - Which of the following muscle proteins is not...Ch. 10 - Prob. 6TYRCh. 10 - Prob. 7TYRCh. 10 - Single-unit smooth muscle cells can stimulate each...Ch. 10 - Prob. 9TYRCh. 10 - Prob. 10TYRCh. 10 - Prob. 11TYRCh. 10 - Prob. 12TYRCh. 10 - Prob. 13TYRCh. 10 - Prob. 14TYRCh. 10 - Prob. 15TYRCh. 10 - Prob. 16TYRCh. 10 - Prob. 17TYRCh. 10 - Prob. 18TYRCh. 10 - Prob. 19TYRCh. 10 - A wave of contraction passing along the esophagus...Ch. 10 - Prob. 1BYMVCh. 10 - Prob. 2BYMVCh. 10 - Prob. 3BYMVCh. 10 - State a meaning of each word element and give a...Ch. 10 - Prob. 5BYMVCh. 10 - Prob. 6BYMVCh. 10 - Prob. 7BYMVCh. 10 - Prob. 8BYMVCh. 10 - Prob. 9BYMVCh. 10 - Prob. 10BYMVCh. 10 - Prob. 1TOFCh. 10 - Prob. 2TOFCh. 10 - Prob. 3TOFCh. 10 - Determine which five of the following statements...Ch. 10 - Prob. 5TOFCh. 10 - Prob. 6TOFCh. 10 - Prob. 7TOFCh. 10 - Prob. 8TOFCh. 10 - Prob. 9TOFCh. 10 - Prob. 10TOFCh. 10 - In a baseball game, the pitcher hits the batter in...Ch. 10 - Prob. 2TYCCh. 10 - Prob. 3TYCCh. 10 - Prob. 4TYCCh. 10 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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