Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
Question
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Chapter 10, Problem 10.13PS

(a)

Summary Introduction

To determine: The electron donor and an electron acceptor in the given system along with the most likely pathway of electron transport in a system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.

Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.

(b)

Summary Introduction

To determine: The number of moles of ATP that would be formed per mole of β-hydroxybutyrate oxidized, and write a balanced equation for the reaction that occurs in the given system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.

Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.

(c)

Summary Introduction

To explain: The purpose of adding cyanide to the system and the possible results if cyanide was not added in the reaction system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.

Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.

(d)

Summary Introduction

To explain: Whether the enzymes of the citric acid cycle would be active in the assay where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide, giving the reason for the same.

Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.

(e)

Summary Introduction

To explain: The importance of β-ketobutyrate not being metabolized in the given system, along with the possible effects if lactate was used as an oxidizable substrate instead of β-hydroxybutyrate as lactate and β-ketobutyrate are similar in structure.

Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.

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