The length of an E. coli DNA molecule, compared with the length of the cell dimensions, and the reason behind the packaging of DNA into the cell. Introduction: The genetic information stored in the cell organelles (mitochondria and nuclei) that decides the development of an organism is genetic material. The genetic material also called DNA (deoxyribonucleic acid).

Question

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2

Answer 1

Question

Chapter 1, Problem 3P

(a)

Summary Introduction

To determine: The length of an E. coli DNA molecule, compared with the length of the cell dimensions, and the reason behind the packaging of DNA into the cell.

Introduction:

The genetic information stored in the cell organelles (mitochondria and nuclei) that decides the development of an organism is genetic material. The genetic material also called DNA (deoxyribonucleic acid).

(b)

Summary Introduction

To determine: The maximum number of the proteins that can be encoded by an E. coli DNA molecule when protein has average of 400 amino acids.

Introduction:

The genetic information stored in the cell organelles (mitochondria and nuclei) that decides the development of an organism.

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